Bioinformatics-driven, rational engineering of protein thermostability

Bioinformatics-driven, rational engineering of protein thermostability.

A longstanding goal in protein engineering is to identify specific sequence changes that endow proteins with desired functional properties. As opposed to traditional rational and random protein engineering techniques, we have employed a bioinformatic approach to identify specific sequence changes that influence key functional properties of a protein within a defined superfamily. Specifically, w...

Structural Bioinformatics for Protein Engineering

Bioinformatics-driven discovery of rational combination for overcoming EGFR-mutant lung cancer resistance to EGFR therapy

MOTIVATION Non-small-cell lung cancer (NSCLC) is the leading cause of cancer death in the United States. Targeted tyrosine kinase inhibitors (TKIs) directed against the epidermal growth factor receptor (EGFR) have been widely and successfully used in treating NSCLC patients with activating EGFR mutations. Unfortunately, the duration of response is short-lived, and all patients eventually relaps...

Engineering thermostability in serine protease inhibitors.

Unlike most globular proteins, the native form of serine protease inhibitors (serpins) is strained. Previous studies of human alpha(1)-antitrypsin, a prototype plasma serpin, revealed that various unfavorable interactions, such as overpacking of side chains, buried polar groups and cavities, are the structural basis of the strain. The local strain could be relieved by various stabilizing single...

Bioinformatics & Knowledge Engineering

Two softwares used for sequence alignment and searches are BioJava and BioPerl Comparison between these two tolls is shown. There are several software engineering issues such as software reuse, component-based software engineering, cleanroom engineering. We are discussing here software reuse (reusable objects). What kind and amount of bimolecular data is available and how to encode that data an...